PeproGrow-hESC is a serum- and phenol red-free medium of a complete, chemically-defined formulation designed for feeder-free maintenance and expansion of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) using BD Matrigel™ as a surface-coating matrix. This medium is intended for the culturing of hESCs and hiPSCs in the undifferentiated, pluripotent state (SSEA4+/Oct4+), and demonstrates less than 15% spontaneous differentiation as indicated by flow cytometry (Figure 1). The proprietary formulation of the medium includes relevant growth factors, such as FGF2 (FGF-basic), but does not contain the insulin found in the majority of other hESC/hiPSC media currently available on the market. PeproGrow-hESC, was designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University.